3D kidney mesenchyme culture

Characterization of the culture

3D_mesenchyme

Image panel describing the 3D kidney mesenchyme culture. Bright field image of spheroids in culture (left), immunofluorescence images represent progenitor (center), and differentiated (right) states of the culture.

Take a look at a video of animated rendering of differentiated kidney mesenchymes spheroid 3D structure in my youtube channel.

Culture method

scheme1

Embryonic kidney mesenchyme (orange) was separated by microdissection from ureteric epithelium (red), enzymatically dissociated to single cells and plated on a 3D matrix in 96-well plate.

Publications

Dapkunas A, Rantanen V, Gui Y, Lalowski M, Sainio K, Kuure S, Sariola H. Simple 3D culture of dissociated kidney mesenchyme mimics nephron progenitor niche and facilitates nephrogenesis Wnt-independently. Sci Rep. 2019 Sep 17;9(1):13433. doi: 10.1038/s41598-019-49526-x. PMID: 31530822; PMCID: PMC6748995.

Ihermann-Hella A, Hirashima T, Kupari J, Kurtzeborn K, Li H, Kwon HN, Cebrian C, Soofi A, Dapkunas A, Miinalainen I, Dressler GR, Matsuda M, Kuure S. Dynamic MAPK/ERK Activity Sustains Nephron Progenitors through Niche Regulation and Primes Precursors for Differentiation. Stem Cell Reports. 2018 Oct 9;11(4):912-928. doi: 10.1016/j.stemcr.2018.08.012. Epub 2018 Sep 13. PMID: 30220628; PMCID: PMC6178244.